Figure 3

Cell cycle analysis of lung cancer cell lines grown as monolayer or MTS. Cell lines INER-37 or INER-51 as monolayer (A, C) or MTS (B, D) respectively, were disassociated with 0.25% trypsin, washed, and stained with propidium iodide for DNA content analysis as described in Material and methods. Flow cytometry was then used to generate cell cycle profiles. Each flow cytometry plot depict the mean percentage of G1 (2n), S and G2/M (4n) fraction population. Each value represents the mean of at least two experiments.