Figure 3

Down-regulation the expression of lncRNA DLX6-AS1 decreased the DLX6 protein levels. a Using qRT-PCR analysis in A549 cells, the expression levels of lncRNA DLX6-AS1 in si-LncRNA group were significantly lower, compared to NC and Blank groups (*P< 0.05). b DLX6 mRNA expression in si-LncRNA group was also lower than in the NC and Blank groups in A549 cells by qRT-PCR (*P< 0.05). c Western blotting analysis showed that DLX6 protein levels in si-LncRNA group were also lower than NC and Blank groups in A549 cells (*P< 0.05). β-actin was used as an endogenous reference. d qRT-PCR analysis verified that the expression level of lncRNA DLX6-AS1 in si-LncRNA group was significantly lower, compared to NC and Blank groups in H1650 cells (*P< 0.05). e DLX6 mRNA expression in si-LncRNA group was also lower than in NC and Blank groups in H1650 cells by qRT-PCR (*P< 0.05). f Western blotting analysis showed that DLX6 protein levels in si-LncRNA group were also lower than in the NC and Blank groups in H1650 cells (*P< 0.05). si-LncRNA group: cells transfected with siRNA targeting lncRNA DLX6-AS1; NC group: cells transfected with negative control siRNA; Blank group: non-transfected cells.