Figure 2

Confocal microscopy of A20 cells after treatment with labeled siGLORNAi and peptide-siGLORNAi. A20 cells were incubated with siGLORNAi for 4 hours. After 3 washes, cells were analyzed at confocal microscopy. (A). Upper row: untreated A20 cells. Middle row: A20 cells after treatment with siGLORNAi alone. Lower row: A20 cells transfected with siGLORNAi by using Lipofectamine 2000. Peptide-siGLORNAi complexes were incubated with A20 cells for 4 hours and after 3 washes, cells were analysed at confocal microscopy. (B). Upper row and Middle row: images at low and high magnification, respectively of A20 cells treated with A20-36-9R-siGLORNAi. Lower row: A20 cells treated with RND-9R-siGLORNAi. (C) Upper row and Middle row: images at low and high magnification, respectively of A20 cells treated with 5R-A20-36-5R-siGLORNAi. Lower row: A20 cells treated with 5R-RND-5R-siGLORNAi. a, e and i) Light microscopy of A20 cells; b, f and j) Membrane staining; c, g and k) Nuclear staining; d, h and l) fluorescence of siGLORNAi.