Figure 3

Down-regulation of GAPDH expression in NIH/3T3 and A20 cells. siRNAGAPDH was transfected with Lipofectamine 2000 at 37°C for 30 and 72 hours in (A) NIH/3T3 and (B) A20 cells. After incubation, cells extracts were analysed by western blot with anti-GAPDH antibody. (A) Lanes: C, untreated cells; 1 and 2 cells treated for 30 hours with 100 and 250 pmoles of siRNAGAPDH, respectively; 3 and 4, cells treated for 72 hours with 100 and 250 pmoles of siRNAGAPDH, respectively. (B) Lanes: C, untreated cells; 1 and 2 cells treated for 30 hours with 100 pmoles of siRNAGAPDH and siRNASCR, respectively; 3 and 4, cells treated for 72 hours with 100 pmoles of siRNAGAPDH and siRNASCR, respectively. Data from three experiments are reported as the mean ± SD. * p < 0.05 compared to control.