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Fig. 1 | Cancer Cell International

Fig. 1

From: Inhibitor of caspase-activated DNase expression enhances caspase-activated DNase expression and inhibits oxidative stress-induced chromosome breaks at the mixed lineage leukaemia gene in nasopharyngeal carcinoma cells

Fig. 1

Hydrogen peroxide (H2O2) induces MLL gene cleavage in NPC cells. SUNE1 cells were treated with either 0 μM, 10 μM, 50 μM, 100 μM, 500 μM, 1 mM or 10 mM of H2O2 for 20 h (lanes 1–7 respectively). Genomic DNA was extracted and modified for IPCR as described in Materials and Methods. DNA was digested with Msc I during the modification. Side arrow shows the 2.2 kb band resulting from amplification of the intact MLL gene. Side bracket shows bands smaller than 2.2 kb resulting from amplification of cleaved MLL gene. M1 represents 1 kb DNA marker

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