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Fig. 2 | Cancer Cell International

Fig. 2

From: Inhibitor of caspase-activated DNase expression enhances caspase-activated DNase expression and inhibits oxidative stress-induced chromosome breaks at the mixed lineage leukaemia gene in nasopharyngeal carcinoma cells

Fig. 2

Transient transfection with normal and mutant CAD do not enhance H2O2-induced MLL gene cleavage. a Microscopic morphology of transiently transfected SUNE1 cells. SUNE1 cells were transfected with either the vector pTracer, T; pTracer-hCAD, h; pTracer-mCAD, m; or pTracer-hCAD (k157Q), k. A mock transfection (no DNA), N, was included as control. Transfected cells were either treated with 50 μM of H2O2 for 8 h at 17 h post-transfection, or left untreated. Observation was then carried out with light microscopy and fluorescence microscopy. b Nested IPCR detection of MLL gene cleavage. SUNE1 cells were transfected with either the vector pTracer, T (Lanes1 and 6), pTracer-hCAD, h (Lanes 2 and 7), pTracer-mCAD, m (Lanes 3 and 8) or pTracer-hCAD (k157Q), k (Lanes 4 and 9). A mock transfection (no DNA), N (Lanes 5 and 10) was included as control. The cells were either non-treated (Lanes 1–5) or treated with 50 μM of H2O2 for 8 h at 17 h post-transfection. Genomic DNA extracted was modified for IPCR as described in Materials and Methods. Side arrow shows the 2.2 kb band resulting from amplification of the intact MLL gene. Side bracket shows bands smaller than 2.2 kb resulting from amplification of cleaved MLL gene. M1 represents 1 kb DNA marker. M2 represents 100 bp DNA marker

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