Fig. 3From: Inhibitor of caspase-activated DNase expression enhances caspase-activated DNase expression and inhibits oxidative stress-induced chromosome breaks at the mixed lineage leukaemia gene in nasopharyngeal carcinoma cellsICAD expression enhances CAD expression. Two sets of SUNE1 cells were transiently transfected with vector pTracer (Lane 2), pcDNA (Lane 3), pTracer-hCAD (Lane 4), pcDNA-mICAD-L (Lane 5), or cotransfection with pTracer-hCAD/pcDNA-mICAD-L (Lane 6) and pTracer-hCAD (K157Q)/pcDNA-mICAD-L (Lane 7). A mock transfection (No DNA) was included as control (Lane 1). Transfected cells were either not treated (Panel a) or treated with 50 μM of H2O2 for 6 h at 17 h post-transfection (Panel b). Protein was extracted as detailed in Materials and Methods. Expression of CAD, ICAD and GAPDH was each analysed by anti-CAD, anti-ICAD and anti-GAPDH respectivelyBack to article page