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Fig. 3 | Cancer Cell International

Fig. 3

From: GSK3β mediates pancreatic cancer cell invasion in vitro via the CXCR4/MMP-2 Pathway

Fig. 3

GSK3β promotes PANC1 human pancreatic cancer cells invasion. Transwell invasion assay was employed to determine the effect of GSK3β on cell invasion. Stable cell clones of PANC1 cells overexpression or suppression of GSK3β were selected. 200 μl cell suspension maitained in serum-free DMEM/0.1 % BSA were added to the matrigel-coated upper compartment, the DMED/10%FBS medium was added to the lower compartment, and the plates were incubated for 48 h at 37 °C. a After incubation for 48 h, the upper surface of the filter was scrubbed free of cells, the filter was fixed and stained and the lower surface was photographed (200× original magnification). b Quantification of A. Results are expressed as the mean ± SD; *p < 0.05 compared to control cells

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