Fig. 2From: MicroRNA-24 inhibits growth, induces apoptosis, and reverses radioresistance in laryngeal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis proteinEffects of miR-24 expression on growth, colony formation, and apoptosis in LSCC. a qRT-PCR of miR-24 expression in mock or stably transfected Hep-2 and AMC-HN-8 cells. U6 was used as an internal control. b MTT analysis of Hep-2 and AMC-HN-8 growth following stable transfection with pGCMV/miR-NC or pGVMV/miR-24, respectively. c Colony formation assay was performed as described in Methods. d Flow cytometric analysis of apoptosis in Hep-2 and AMC-HN-8 stably transfected with pGCMV/miR-NC or pGVMV/miR-24, respectively. e Western blot detection of c-caspase-3, total caspase-3, c-PARP, and total PARP in the stably transfected Hep-2 and AMC-HN-8 cells. GAPDH was used as an internal control. Each experiment was performed at least in triplicate. *P < 0.05, **P < 0.01 vs controlBack to article page