Skip to main content
Fig. 4 | Cancer Cell International

Fig. 4

From: MicroRNA-24 inhibits growth, induces apoptosis, and reverses radioresistance in laryngeal squamous cell carcinoma by targeting X-linked inhibitor of apoptosis protein

Fig. 4

miR-24 binds to the 3′-UTR of XIAP mRNA. a A human XIAP/3′-UTR fragment containing wild-type or mutant miR-24-binding sequence was cloned downstream of the luciferase reporter gene in pLUC-luc. b pLUC-luc vector contains XIAP/3′-UTR-wt or XIAP/3′-UTR-mut and pGCMV/miR-24 or pGCMV/miR-NC were co-transfected into Hep-2 cells, and cell lysates prepared at 48 h for measuring luciferase activity, which was normalized to Renilla luciferase activity. c Western blot detection of XIAP protein expression in the stably transfected Hep-2 and AMC-HN-8 cells. GAPDH was used as an internal control. Each experiment was performed at least in triplicate. *P < 0.05, **P < 0.01 vs control

Back to article page