Table 1 Primer sequences and reaction conditions for genotyping IL-18 polymorphisms
Polymorphisms | Primer sequence | Annealing temperature | Restriction enzyme | Product size (bp) |
|---|---|---|---|---|
−607C/A | F:5′-TCAGTGGAACAGGAGTCCAT-3′ | 50°C | DraI | AA: 109 + 41 |
R:5′-GCAGAAAGTGTAAAAATTTTT-3′ | CC: 150 | |||
AC: 109 + 41 + 150 | ||||
−137G/C | F:5′-AGGTGCTTTCTTAAAGTCAGA-3′ | 50°C | HinfI | GG: 107 + 42 |
R:5′-AATATCACRATTTTCATGGAA-3′ | CC: 149 | |||
GC: 107 + 42 + 149 |