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Fig. 3 | Cancer Cell International

Fig. 3

From: Effects of the aqueous extract of a Tibetan herb, Rhodiola algida var. tangutica on proliferation and HIF-1α, HIF-2α expression in MCF-7 cells under hypoxic condition in vitro

Fig. 3

Induction of apoptosis and/or necrosis assessed by flow cytometry. Externalization of phosphotidylserine (PS) to the outer leaflet from the inner leaflet of the plasma membrane is a hallmark of early apoptosis. The FITC labeled Annexin V binds to PS in presence of calcium ions, resulting in green fluorescence of apoptotic cells. In later stages of apoptosis or necrosis, PI enters the cells and bind to cellular DNA, resulting in red and intense green fluorescence with Annexin V. As shown in the Fig. 3, cells treated with the lower concentration showed the present of Annexin V (+) (lower right quadrant) indicating the induction of early apoptosis. However, at higher concentration, there was a prominent rise of Annexin V(+)/PI (+) indicative of late apoptosis/necrosis.

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Cancer Cell International

ISSN: 1475-2867

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