Fig. 5

Effects of hypoxia and Rhodiola algida var. tangutica on HIF-1α, HIF-2α protein expression in MCF-7 cells cultured detected by Western blotting. a Western blots of cultured MCF-7 breast cancer cells exposed to hypoxia for 48 h in the absence or presence of various concentrations of Rhodiola algida var. tangutica (45, 90, 180, 225, 360 μg/mL). Cell lysates were analyzed by Western blotting with antibodies as indicated above. GAPDH protein expression was used as a control. b, c Bars show the relative expression levels of HIF-1α, HIF-2α (optical density of HIF-1α, HIF-2α normalized against GAPDH expression). Data are mean ± SEM of three identical experiments. N48 h indicates exposure to normoxia for 48 h; H48 h, exposure to hypoxia for 48 h. ^◆P < 0.01 vs. N48 h group, ^★P < 0.05 vs. H48 h group.