siRNA of S100A7 transfected cells can down-regulate the expression of S100A7 protein level through AKT signaling. a Flow cytometric analysis of sub G0/G1 cell population of JMAR and Tu167 cells. b Western blotting of S100A7 protein in JMAR cells transfected with siRNA of S100A7. The JMAR cell line was pre-incubated with or without LY294002 for 24 h in suspension culture prior to determination of apoptotic fraction of cells by flow cytometry. c, d Phosphorylation of Akt and expression of S100A7 by western blot.