Fig. 5

Effect of S100A7 expression on anoikis in head and neck cancer cells. a Western blot analysis of vector and stable clones of S100A7 expression was moderate (C1) and higher (C2) level. The blot was reprobed with a β-actin as a loading control. b S100A7 mRNA expression in the stable clones. c Expression of S100A7 by immunofluorescence microscopy in the stable clones. d Detached cells were suspended in a 15 ml tube and rotated in the incubator at different time as described in the “Methods”, and flow cytometry assessed sub G0/G1 cells. Results shown are representative of three experiments.