Effect of S100A7 expression on Soft agar colony formation and tumorigenicity of head and neck cancer cells. a Anchorage independent growth of TU167 cells stably transfected with different level of S100A7 expression i.e. C1 and C2 clones along with vector-transfected. Results were representative photographs of soft agar colonies (n = 3). b Survival curve of nude mice orthotopically implanted with vector (pcDNA3.1) or S100A7 clones (C1 and C2) tumors. Nude mice were orthotopically implanted in the tongue with 1 × 106 Vector or C1 or C2 S100A7 stable cells and observation showed weight loss with time. Animals losing more than 25% of their initial body weight were sacrificed. All the vector injected animals survived greater than 25 days without significant weight loss and therefore were sacrificed at that time point. c IHC of S100A7expression in FFPE tumor specimens from vector, C1, C2 orthotopically injected nude mice.