Table 1 Different types of medium for primary skeletal muscle cell culture

Plating medium

Dulbecco’s modified Eagle’s medium (DMEM)

10% horse serum (HS)

0.5% chick embryo extract (CEE)

1% penicillin–streptomycin (P/S)

1% l-glutamine

Proliferation medium

DMEM

10% HS

10% fetal bovine serum (FBS)

1% CEE

1% P/S

1% l-glutamine

Differentiation medium

DMEM

2% FBS

0.5% CEE

1% P/S

1% l-glutamine