Fig. 1
From: Etoposide induces cell death via mitochondrial-dependent actions of p53
Concentration-dependent etoposide-induced apoptosis. a Flow cytometry analysis of MEFs was performed to determine the percentage of cells with DNA content below the threshold for cells in G1, following 18 h treatment with 1.5, 15 or 150 µM etoposide. Data shown are mean + SD from seven independent experiments with three replicates each. b MEFs were treated with 1.5, 15 or 150 µM etoposide for 3, 6 or 18 h. Con indicates extract from untreated cells. Total cell proteins were probed with anti-Caspase-3 antibody. Vinculin was used as the loading control. Figures are representative of three independent experiments showing similar results.