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Fig. 2 | Cancer Cell International

Fig. 2

From: Etoposide induces cell death via mitochondrial-dependent actions of p53

Fig. 2

Etoposide effect on p53-mediated transcriptional events. a MEFs were treated with 15 µM of etoposide (upper panel) or 1.5 µM of etoposide (lower panel) for 1, 3, 6 or 18 h. Total cell proteins were probed with anti-PUMA antibody. Vinculin or actin was used as the loading control. b MEFS were treated with 15 µM of etoposide (upper panel) or 1.5 µM of etoposide (lower panel) for 30 min, 1, 3, 6 or 18 h. Total RNA was isolated and reverse transcribed into cDNA. The expression of p21CIP1/WAF1 was determined by RT-PCR using specific primers. β-actin was used a loading control. Figures are representative of three independent experiments.

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