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Fig. 3 | Cancer Cell International

Fig. 3

From: Etoposide induces cell death via mitochondrial-dependent actions of p53

Fig. 3

Effect of PFT-α on etoposide-induced apoptosis. a MEFs, pre-treated with 30 μM PFT-α followed by 18 h treatment with 1.5, 15 or 150 µM etoposide, were analyzed by flow cytometry to determine percentage of cells having sub-G1 DNA content. Cells were treated in parallel with UVC and analyzed after 18 h. Control indicates normally proliferating cells. Columns represent percentage of cells having sub-G1 DNA content, as analyzed by flow cytometry. Data are mean + SD of three independent experiments with three replicates each. b mRNA was extracted from untreated (0 h), 6 and 18 h treatment with 15 µM of etoposide (black bars) or pre-treatment with PFT-α followed by etoposide (open bars). The relative expression of p21cip1/waf1 was determined by qRT-PCR (mean ± SD from three independent experiments). The values of p21cip1/waf1 were normalized to β-actin. c, d Parallel studies used 1.5 or 15 µM etoposide. Sub-G1 population of MEFs was measured as in A for cells that were untreated (Control), pre-treated with 30 μM PFT-α followed by etoposide for 3 h (PFT-α-Etop 3 h), etoposide alone for 19 h (Etop 19 h), pre-treated with PFT-α followed by etoposide for 19 h (PFT-α-Etop 19 h), washed after 3 h of co-treatment, followed by further incubation for 18 h (PFT-α-Etop washed) and washed after 3 h, and PFT-α was added back for 18 h (PFT-α-Etop + PFT-α). Columns represent percentage of cells having sub-G1 DNA content; representative of three experiments with similar results.

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