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Fig. 4 | Cancer Cell International

Fig. 4

From: Etoposide induces cell death via mitochondrial-dependent actions of p53

Fig. 4

Effect of PES on etoposide-induced cell death. a MEFs were either untreated (Con) or treated with etoposide (Etop) for 6 h, or pre-treated with 10 μM of PES followed by 1.5 μM of etoposide for 6 h. Mitochondrial extracts were immunoprecipitated with anti-p53 antibody and probed for Bcl-xL. The first lane (IP Con IgG) represents mitochondrial extracts from untreated (Con) cells immunoprecipitated with rabbit IgG alone Input p53 represents the starting material unbound to the Agarose G beads, probed for the presence of p53. b MEFs were treated with either 1.5 µM etoposide alone or pre-treated with 10 μM of PES and followed by 1.5 µM etoposide for various times. Columns represent percentage of cells having sub-G1 DNA content. A one-way ANOVA was carried out to compare the treatment groups. Post hoc comparison using the Tukey HSD test indicated significant inhibitory effect of PES treatment on the percentage of cells having sub-G1 DNA content. Data shown are mean + SD from 3 independent experiments with three replicates each. c Analysis of MEFs was done as in B; cells were pre-treated with 10 μM PES followed by 18 h treatment with 1.5, 15 or 150 µM etoposide. Cells were treated in parallel with UVC and followed for 18 h. Statistical analysis was as in b. Data are mean + SD of three repeat analyses.

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