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Fig. 5 | Cancer Cell International

Fig. 5

From: Etoposide induces cell death via mitochondrial-dependent actions of p53

Fig. 5

Effect of PES on cell cycle events. a Representative flow cytometry profiles of MEFs, with control (no treatment), treatment with 1.5 µM etoposide for 18 h, pre-treatment with 30 μM of PFT-α followed by 18 h treatment with 1.5 µM etoposide or pre-treatment with 10 μM of PES followed by 18 h treatment with 1.5 µM etoposide. Percentage of cells having sub-G1 DNA content is indicated. Data shown are representative of six experiments with similar results. b Columns represent distribution of cells in G1, S and G2/M phases of cell cycle, as analyzed by flow cytometry. Data are mean + SD of three independent experiments with three replicates each. c MEFs were treated for various times with 15 µM of etoposide either alone or pre-treated with 10 μM of PES. Total RNA was isolated and reverse transcribed into cDNA. The expression of p21cip1/waf1 was determined using specific primers in RT-PCR. β-actin was used as a loading control. Representative of three independent experiments. d MEFs were untreated (Con) or treated with 1.5 µM of etoposide for 3 or 6 h alone or pre-treated with 10 μM PES. Total cell proteins were probed with anti-Ser345 CHK1, anti-CHK1, anti-phospho-Tyr 15 CDK1, anti-CDK1, Cyclin B1 antibodies, and anti-vinculin as the loading control.

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