Fig. 6

Effect of drugs on p53 acetylation and complex formation. a MEFs were either left untreated (Con) or treated with etoposide (Etop) alone or in combination with either PFT-α or PES for 3 h. Total cell extracts were prepared. The blot was probed with anti-Lys373/382 p53 antibody or anti-p53 as a loading control. b MEFs were either untreated (Con) or treated with etoposide (Etop) for 3 h, or pre-treated with either 30 µM of PFT-α or 10 μM of PES followed by 1.5 μM of etoposide for 3 h. Total cell extracts were immunoprecipitated with anti-p53 antibody and probed for HDAC1 and Mdm2. The first lane (IP Con IgG) represents PES and etoposide-treated extracts immunoprecipitated with rabbit IgG alone to indicate position of the IgG heavy chain (IgGH). Input Mdm2 represents the starting material unbound to the Agarose G beads, probed for the presence of Mdm2.