Fig. 4

Cthrc1 was identified as a target of miR-30b in A549 and Calu-3 cells. a Bioinformatics analysis showed the putative binding sequence between miR-30b and Cthrc1 3′UTR. b, c Western blotting analysis of Cthrc1 expression in transfected A549 and Calu-3 cells. GAPDH was used as an endogenous control. d, e Luciferase activity determined 48 h after transfection in A549 and Calu-3 cells. In cells co-transfected with mutant-type 3′UTR of Cthrc1, no difference was found between the relative luciferase activity of cells co-transfected with miR-30b and that of the Scramble group. However, in cells co-transfected with wild-type 3′UTR, the relative luciferase activity of the cells co-transfected with miR-30b was significantly lower compared with the Scramble group. miR-30b cells transfected with miR-30b mimics; Scramble cells transfected with scrambled miR-30b negative control; Blank non-transfected cells