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Fig. 5 | Cancer Cell International

Fig. 5

From: PDK1-mTOR signaling pathway inhibitors reduce cell proliferation in MK2206 resistant neuroblastoma cells

Fig. 5

Effect of AZD8055 (AZD), mTOR inhibitor, in MK-2206-resistant sublines compared with non-resistant cells. a Indicated cell lines were treated with AZD at indicated concentrations, with/without MK-2206 (5 μM) in RPMI1640 + 10 % FBS. Cell growth was evaluated as cell numbers at 72 h, and it was repeated three times. Data are expressed as the mean (±SD). b The effect of AZD on cell cycle phase distribution in LAN-1 and LAN-MK. Indicated cells were treated with AZD (50 nM) with/without MK-2206 (5 μM) in RPMI1640 with 10 % FBS for 12 h followed by analysis of cell cycle phase distribution, as introduced in methods and material. Cells were stained with PI for 30 min followed by FACScan flow cytometer. c IC50 of AZD of indicated cell lines

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