Table 1 Primer used for RT-PCR
Gene name | Primer sequence | Product size (bp) | Accession no. | Annealing temperature | Number of cycles | |
|---|---|---|---|---|---|---|
OCT 4 | S | 5′-CGT GAA GCT GGA GAA GGA GAA GCT-3′ | 245 | AF268617 | 60 | 34 |
A | 5′-CAA GGG CCG CAG CTC ACA CAT GTT C-3′ | |||||
NANOG | S | 5′-CAA AGG CAA ACA ACC CAC TT-3′ | 394 | NM_024865 | 60 | 34 |
A | 5’-ATT GTT CCA GGT CTG GTT GC-3′ | |||||
SOX 2 | S | 5′-AAC CCC AAG ATG CAC AAC TC-3′ | 100 | BC13923.2 | 60 | 34 |
A | 5′-CGG GGC CGG TAT TTA TAA TC-3′ | |||||
EpCAM | S | 5′-CTG GCC GTA AAC TGC TTT AG-3′ | 182 | BC014785.1 | 60 | 34 |
A | 5′-AGC CCA TCA TTG TTC TGG AG-3′ | |||||
Connexin 32 | S | 5′-GTT TGA GGC CGT CTT CAT GT-3′ | 188 | BC039198.1 | 60 | 34 |
A | 5′-CCA CAT TGA GGA TGA TGC AG-3′ | |||||
Connexin 43 | S | 5′-GGA CAT GCA CTT GAA GCA GA-3′ | 103 | BC026329.1 | 60 | 34 |
A | 5′-GAT GAT GTA GGT TCG CAG CA-3′ | |||||
GAPDH | S | 5′-TCC ATG ACA ACT TTG TGA TC-3′ | 452 | NM_002046 | 55 | 34 |
A | 5′-TGT AGC CAA ATT CGT TGT TA-3′ |