Table 2 Sequences of primers and probes for real-time PCR (TaqMan method)
From: Analysis of the expression of PHTF1 and related genes in acute lymphoblastic leukemia
Primer/probe | Sequence |
|---|---|
BCL11B-F | 5′-CACCCCCGACGAAGATGACCAC-3′ |
BCL11B-R | 5′-CGGCCCGGGCTCCAGGTAGATG-3′ |
BCL11B-P | 5′-FAM-TCACCCACGAAAGGCATCTGTCCCAAGCA-TAMRA-3′ |
β2M-F | 5′-CTCGCGCTACTCTCTCTTTCT-3′ |
β2M-R | 5′-TACATGTCTCGATCCCACTTAACTAT-3′ |
β2M-P | 5′-FAM-CTCACGTCATCCAGCAGAGAATGGAAAGTCA-TAMRA-3′ |
PHTF1-F | 5′-GGAAAGTGATGACTGCAGAAACC-3′ |
PHTF1-R | 5′-AACACCATTCATTCGCTTTGG-3′ |
PHTF1-P | 5′-FAM-CCTGCTTGTTCACATGCACAGGTGC-TAMRA-3′ |
FEM1B-F | 5′-CACTCCATCATCATTAGCCTAGTTGA-3′ |
FEM1B-R | 5′-TGTACTTTTGTCTAGCGGAGTCTTATTCT-3′ |
FEM1B-P | 5-FAM-CCGGAGCTCACACTGACATGACGAATAA-TAMRA-3′ |
APAF1-F | 5′-TGCGCTGCTCTGCCTTCT-3′ |
APAF1-R | 5′-CATGGGTAGCAGCTCCTTCTTC-3′ |
APAF1-P | 5′-FAM-TGAGCTTCTTCATTTGTGTGCTCCGCT-TAMRA-3′ |