Fig. 5

Modulation of cell cycle profile and cell cycle-regulatory proteins in jacaric acid-treated PU5-1.8 cells. a PU5-1.8 cells were incubated with different concentrations of jacaric acid at 37 °C for 72 h. Cells treated with ethanol acted as the control. The DNA content was analyzed by PI staining and flow cytometry. Cell cycle distribution of the samples was calculated by Modfit LT 3.0 programme. The results were expressed as mean ± SE. *p < 0.05. b PU5-1.8 cells were incubated with 4 μM jacaric acid (Lane 2) and 8 μM jacaric acid (Lane 3) at 37 °C for 72 h. Cells treated with ethanol (Lane 1) acted as the control. Protein expression levels of CDK2, cyclin E, p21, p27 and pp53 were assayed by Western blotting with β-actin protein as an internal control. c–g The relative protein expression levels of CDK2, cyclin E, p21, p27 and pp53 compared to β-actin were quantified. Results represent mean ± SE. *p < 0.05; ***p < 0.001