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Fig. 7 | Cancer Cell International

Fig. 7

From: Induction of atypical cell death in thyroid carcinoma cells by the indirubin derivative 7-bromoindirubin-3′-oxime (7BIO)

Fig. 7

Absence of conversion of LC3B-I after treatment with 3 µM 7BIO indicating no involvement of autophagic processes in 7BIO-mediated cell death. Western blot analyses of vehicle-treated and 7BIO-treated thyroid carcinoma cell lines using LC3B antibody. As a positive control for autophagy induction, HepG2 cells treated with 0.2 µM obatoclax were included. Equal protein loading was ensured by in-gel protein staining (see “Methods”)

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