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Table 2 Distribution of cell cycle phases in vehicle-treated and 7BIO-treated cells (24 h, 3 µM)

From: Induction of atypical cell death in thyroid carcinoma cells by the indirubin derivative 7-bromoindirubin-3′-oxime (7BIO)

Cell line

Type

Status

% subG1

% G1

% G2/M

% S

FTC236

Follicular

Untreated

0.2 ± 0.1

38.4 ± 4.3

18.7 ± 0.8

42.9 ± 3.0

7BIO 24 h

43.6 ± 4.7*

48.7 ± 4.0*

6.2 ± 0.9*

45.1 ± 5.1

ML1

Follicular

Untreated

1.2 ± 0.2

39.4 ± 5.0

29.5 ± 2.4

31.1 ± 3.5

7BIO 24 h

44.0 ± 5.3*

76.8 ± 9.3*

16.9 ± 1.5*

6.3 ± 0.7*

BHT101

Papillary

Untreated

0.5 ± 0.2

51.3 ± 4.3

17.4 ± 1.1

31.4 ± 1.5

7BIO 24 h

11.5 ± 2.1*

83.6 ± 6.8*

10.4 ± 0.7*

6.0 ± 1.1*

SW1736

Anaplastic

Untreated

1.2 ± 0.2

36.4 ± 3.7

22.1 ± 1.8

41.5 ± 2.4

7BIO 24 h

12.7 ± 1.6*

48.2 ± 6.1*

18.5 ± 3.4

33.3 ± 3.5*

HTh7

Anaplastic

Untreated

1.0 ± 0.1

52.0 ± 6.4

15.1 ± 1.3

32.9 ± 2.8

7BIO 24 h

13.1 ± 1.5*

63.3 ± 3.9*

17.3 ± 1.5

19.4 ± 0.9*

C643

Anaplastic

Untreated

0.6 ± 0.1

53.8 ± 6.7

12.4 ± 0.7

33.7 ± 1.2

7BIO 24 h

9.3 ± 1.1*

48.7 ± 6.8

5.5 ± 0.3*

45.9 ± 4.5*

  1. Values for G1-, G2/M- and S-phase are determined for the living cells that were not included in the sub-G1-peak
  2. * Significant change (p < 0.05, Student’s t test) compared to controls treated with vehicle