Induction of atypical cell death in thyroid carcinoma cells by the indirubin derivative 7-bromoindirubin-3′-oxime (7BIO)

Table 3 Viability of cells after incubation with 3 µM 7BIO, 5 µM Q-VD-OPh, 2 µM 3-MA or the combinations indicated for 24 h (MTT assay)

Cell line	Origin	7BIO	Q-VD-OPh	7BIO + Q-VD-OPh	3-MA	7BIO + 3-MA
FTC236	Follicular	41.5 ± 4.8*	102.4 ± 6.9	45.2 ± 7.2	94.6 ± 10.4	39.3 ± 7.8
ML1	Follicular	72.3 ± 4.2*	105.1 ± 6.0	70.3 ± 6.8	106.0 ± 7.5	71.6 ± 7.7
BHT101	Papillary	68.3 ± 8.3*	97.4 ± 8.2	71.2 ± 5.3	98.4 ± 5.7	69.4 ± 9.4
SW1736	Anaplastic	39.5 ± 8.2*	95.7 ± 11.4	44.5 ± 9.1	104.6 ± 9.7	42.6 ± 9.5
HTh7	Anaplastic	50.8 ± 6.2*	100.6 ± 7.9	53.5 ± 8.3	106.7 ± 10.3	49.2 ± 7.5
C643	Anaplastic	21.5 ± 4.8*	104.5 ± 9.7	22.9 ± 5.5	99.1 ± 6.9	18.6 ± 7.4

* Significant decrease (p < 0.05, Student’s t test) of 7BIO-treated cells compared to vehicle-treated controls, while treatment with Q-VD-OPh or 3-MA alone had no significant effect compared to vehicle-treated controls. Treatment with a combination of 7BIO and Q-VD-OPh or 7BIO and 3-MA was compared with the 7BIO treatment and had no significant effects

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