Fig. 1
From: STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7
Effects of STAT1 overexpression on SMMC7721 and HepG2 cells growth. a, b SMMC7721 and HepG2 cells were transfected with an expression vector containing STAT1 and empty vector (EV), or they were left untreated, the levels of STAT1 expression were determined by qRT-PCR and western blot assays; c cell proliferation was quantified by MTT assay. STAT1-transfected SMMC7721 and HepG2 cells grew significantly more slowly than EV or untreated cells; d, f apoptosis was monitored by flow cytometry. The percentage of early apoptotic cells was quantified, and the fraction of apoptotic STAT1-transfected SMMC7721 and HepG2 cells were higher than that of EV cells (P < 0.05); e, g cell cycle was monitored by flow cytometry. STAT1-transfected SMMC7721 and HepG2 cells showed a higher proportion of cells in G0/G1 phase (88.17 and 90.87 %) compared with control cells (76.80 and 77.27 %) (P < 0.05)