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Fig. 2 | Cancer Cell International

Fig. 2

From: STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7

Fig. 2

Effects of STAT1 knockdown on SMMC7721 and HepG2 cells growth. a, b, c qRT-PCR and western blot assays were used to determine siRNAs efficiency; d cell proliferation was quantified by MTT assay. STAT1 siRNA-transfected SMMC7721 and HepG2 cells grew significantly faster than control siRNA-transfected cells (P < 0.05); e, g apoptosis was detected by flow cytometry. The percentage of early apoptotic cells was quantified, and the fraction of apoptotic cells was lower in STAT1 siRNA2-transfected cells than in those transfected with control siRNA (P < 0.05); f, h STAT1 knockdown significantly increased the cell cycle progression. The percentage of G0/G1 phase in STAT1 siRNA2 cells (72.61 and 76.38 %) was lower than that of control siRNA cells (80.65 and 84.31 %) (P < 0.05)

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