Table 1 Primer for PCR amplification and siRNAs sequences
From: STAT1 inhibits human hepatocellular carcinoma cell growth through induction of p53 and Fbxw7
Sequencing primers | 5′–3′ |
|---|---|
p53-F primer | CAGATCCCTTAGTTTTGGGTGC |
p53-R primer | GCCTGGAGAGACCTAGACCA |
Fbxw7-F primer | AAAGAGTTGTTAGCGGTTCTCG |
Fbxw7-R primer | CCACATGGATACCATCAAACTG |
HES-1-F primer | CCAAAGACAGCATCTGAGCA |
HES-1-R primer | TCAGCTGGCTCAGACTTTCA |
NF-κB p65-F primer | TCTGCAACTGAAACGCAAGC |
NF-κB p65-R primer | CTCCACAGCTTCTCTTACCTT |
CDK2-F primer | CGGATCTTTCGGACTCTGGG |
CDK2-R primer | GAGAGGGTGAGCCGATTAGG |
CyclinA-F primer | CAGAGGCCGAAGACGAGAC |
CyclinA-R primer | TCAGCTGGCTTCTTCTGAGC |
CyclinD1-F primer | CTGGCCATGAACTACCTGGA |
CyclinD1-R primer | GTCACACTTGATCACTCTCC |
CyclinE-F primer | GTTATAAGGGAGACGGGGAG |
CyclinE-R primer | TGCTCTGCTTCTTACCGCTC |
RITA-F primer | GGGTGGAGAAGGCTAACAGAA |
RITA-R primer | CATCACAGTAAGACGGGGTGT |
GAPDH-F primer | AGGTGAAGGTCGGAGTCAAC |
GAPDH-R primer | CGCTCCTGGAAGATGGTGAT |
siRNA1:STAT1_homo-575 sense | GCUGGAUGAUCAAUAUAGUTT |
siRNA1:STAT1_homo-575 antisense | ACUAUAUUGAUCAUCCAGCTT |
siRNA2:STAT1_homo-647 sense | GCGUAAUCUUCAGGAUAAUTT |
siRNA2:STAT1_homo-647 antisense | AUUAUCCUGAAGAUUACGCTT |
siRNA3:STAT1_homo-1601 sense | GCACCUGCAAUUGAAAGAATT |
siRNA3:STAT1_homo-1601 antisense | UUCUUUCAAUUGCAGGUGCTT |
Control-siRNA-sense | UUCUCCGAACGUGUCACGUTT |
Control-siRNA-antisense | ACGUGACACGUUCGGAGAATT |