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Fig. 2 | Cancer Cell International

Fig. 2

From: The choroid plexus may be an underestimated site of tumor invasion to the brain: an in vitro study using neuroblastoma cell lines

Fig. 2

Fluorescent microscopy study of neuroblastoma cell transmigration through the BCSFB in vitro. Six hours after seeding SH-SY5Y neuroblastoma cells in the upper compartments of the filters, samples were fixed and stained for occludin (tight junction protein, in green), F-actin (cytoskeleton, in pink) and DAPI (nuclei, in blue). Neuroblastoma cells had previously been stained with a fixable CellTracker™ (in yellow). As represented for each set of pictures (upper panels), photographs show representative neuroblastoma cells at different stages of the transmigration process (during, a, b; and after, c) through the epithelial layer formed by HIBCPP cells on the lower side of the filters (two middle panels, en face Apotome® microscopy images showing all channels and selected channels, respectively; lower panels, 3D reconstructions from an apical—a and c—and a lateral point of view—b). Apical and basolateral sides of the barriers are indicated by ‘A’ and ‘B’, respectively. In a the arrowheads indicate the phalloidin-stained filamentous actin cytoskeleton surrounding the transmigrating neuroblastoma cell. In b the arrows point to a possible projection sent by the transmigrating cancer cell through tight junctions. In c arrows indicate the neuroblastoma cell having reached the apical surface of the epithelial barrier. Scale bar as indicated

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