Fig. 1

Effect of cisplatin and/or DMSO on apoptosis induction in EAC cells withdrawn 24 h. after treatment. Apoptosis was analyzed by staining with propiduim iodide (PI, y-axis) and annexin—FITC (x-axis). a EAC cells withdrawn from normal saline (2 ml/kg, i.p.) treated animals. b EAC cells withdrawn from animals treated with 50 % DMSO (2 ml/kg, i.p.). c EAC cells withdrawn from animals treated with cispalin (4.5 mg/kg, i.p.) alone. d EAC cells withdrawn from animals pretreated with 50 % DMSO (2 ml/kg, i.p.) followed by cispatin (4.5 mg/kg, i.p.). The percentage of cells in each quadrant is indicated (R1 necrosis, R2 late apoptosis, R3 live Cells, R4 early apoptosis). The experiment was repeated twice each one in duplicate