Fig. 1

The effects of growth factors on collective migration of HGSOC cell lines. a Treatment with 10 ng/mL HB-EGF, NRG1β, IGF1, or HGF for 48 h impacted wound closure in a subset of HGSOC cell lines. Cells were stained with CellTracker Green, seeded to confluency, and wound area was measured at 0 and 48 h post-treatment. Data presented as the average ± SD, n = 3. *p < 0.05 compared to vehicle. b Representative images of the wounds at time = 0 and 48 h for each cell line and treatment condition. As expected, CellTracker Green intensity had decreased by 48 h, however, the edge of the wound could still be analyzed. Scale bar represents 500 μm