Fig. 4

ESC reversed EMT induced by TGF-β in HepG2 cells by down-regulation of Vimentin and up-regulation of E-cadherin. a Expressions of Vimentin and E-cadherin in HepG2 cells pretreated with different concentrations (1–5 μg/mL) of ESC prior 2 h to TGF-β (5 ng/mL) incubation for 24 h with immune-fluorescence assay. The magnification is ×400. b Western blot results of Vimentin and E-cadherin in HepG2 cells pretreated with different concentrations (0.2–5 μg/mL) of ESC prior 2 h to TGF-β (5 ng/mL) incubation for 24 h. c Western blot results of Vimentin and E-cadherin in HepG2 cells pretreated with 5 μg/mL ESC for different hours (1–4 h) prior to 5 ng/mL TGF-β stimulation. Western blot data presented were representative of those obtained in at least 3 separate experiments. The value of the control cells was set to 1