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Table 3 Migratory behavior of A2058 melanoma cells detected by holographic microscopy

From: Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1)

A2058 Methanol Alizarin (10−5 M) Purpurin (10−5 M)
Avg. Migration (μm)
 Mean 5.347 ± 0.16 8.719 ± 0.25** 22.667 ± 0.82***
 Slope 0.034 ± 6.82E−4 0.056 ± 7.03E−4 0.170 ± 0.01***
 R 0.955 0.981 0.933
 Adj. R-square 0.912 0.963 0.870
Avg. migration directness (migration vs motility)
 Mean 0.110 ± 0.01 0.100 ± 0.01 0.196 ± 0.01**
 Slope −6.40E−4 ± 7.83E−5 −7.14E−4 ± 8.38E−5 −2.30E−4 ± 6.79E−5**
 R 0.468 0.482 0.214
 Adj. R-square 0.215 0.229 0.042
Avg. motility (µm)
 Mean 75.229 ± 2.63 125.627 ± 4.94** 104.172 ± 3.99*
 Slope 0.587 ± 0.01 1.099 ± 0.01*** 0.890 ± 0.01**
 R 0.998 0.997 0.998
 Adj. R-square 0.996 0.995 0.996
Avg. motility speed (µm/h)
 Mean 79.931 ± 1.14 134.900 ± 2.44** 104.630 ± 4.75*
 Slope 0.063 ± 0.01 0.279 ± 0.03*** 0.045 ± 0.06
 R 0.248 0.514 0.042
 Adj. R-Square 0.057 0.261 0.002
  1. Effect of treatment with alizarin (10−5 M) and purpurin (10−5 M)
  2. Data shown in the table represent variables and ± S.D. values calculated for 50 cell/group in 240 consecutive frames
  3. The ‘Mean’ values are expressed as dimensions (μm, μm/h) shown in the sub-headers of the table
  4. The slope values are expressed as dimensionless values to describe the changing rate of Mean values in the 2 h’ time interval of treatments
  5. R and Adj. R-Square represent data of linear regression analysis to describe trends of variables belonging to the parameters calculated
  6. The level of significance is shown as follows: * p < 0.05; ** p < 0.01; *** p < 0.001