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Table 4 Migratory behavior of HT168-M1 melanoma cells detected by holographic microscopy

From: Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1)

HT168-M1 Methanol Alizarin (10−5 M) Purpurin (10−5 M)
Avg. Migration (μm)
 Mean 8.870 ± 3.65 26.651 ± 10.65*** 15.490 ± 6.14***
 Slope 0.052 ± 4.54E−4 0.140 ± 0.01 0.086 ± 0.01
 R 0.991 0.920 0.976
 Adj. R-square 0.982 0.846 0.953
Avg. migration directness (migration vs. motility)
 Mean 0.127 ± 0.01 0.189 ± 0.01** 0.146 ± 0.01
 Slope 6.55E−4 ± 8.10E−5 8.23E−4 ± 7.26E−5 7.52E−4 ± 7.89E−5
 R 0.464 0.524 0.464
 Adj. R-square 0.212 0.346 0.272
Avg. motility (µm)
 Mean 96.346 ± 3.49 184.515 ± 7.38*** 118.951 ± 4.21*
 Slope 0.778 ± 0.01 1.643 ± 0.01 0.937 ± 0.01
 R 0.998 0.999 0.997
 Adj. R-square 0.997 0.998 0.997
Avg. motility speed (µm/h)
 Mean 96.609 ± 1.46 188.513 ± 3.94*** 115.551 ± 2.05
 Slope 0.056 ± 0.02 0.199 ± 0.05 0.033 ± 0.03
 R 0.172 0.227 0.074
 Adj. R-square 0.025 0.047 0.001
  1. Effect of treatment with alizarin (10−5 M) and purpurin (10−5 M)
  2. Data shown in the table represent variables and ± S.D. values calculated for 50 cell/group in 240 consecutive frames
  3. The ‘Mean’ values are expressed as dimensions (μm, μm/h) shown in the sub-headers of the table
  4. The slope values are expressed as dimensionless values to describe the changing rate of Mean values in the 2 h’ time interval of treatments
  5. R and Adj. R-Square represent data of linear regression analysis to describe trends of variables belonging to the parameters calculated
  6. The level of significance is shown as follows: * p < 0.05; ** p < 0.01; *** p < 0.001