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Table 4 Migratory behavior of HT168-M1 melanoma cells detected by holographic microscopy

From: Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1)

HT168-M1

Methanol

Alizarin (10−5 M)

Purpurin (10−5 M)

Avg. Migration (μm)

 Mean

8.870 ± 3.65

26.651 ± 10.65***

15.490 ± 6.14***

 Slope

0.052 ± 4.54E−4

0.140 ± 0.01

0.086 ± 0.01

 R

0.991

0.920

0.976

 Adj. R-square

0.982

0.846

0.953

Avg. migration directness (migration vs. motility)

 Mean

0.127 ± 0.01

0.189 ± 0.01**

0.146 ± 0.01

 Slope

6.55E−4 ± 8.10E−5

8.23E−4 ± 7.26E−5

7.52E−4 ± 7.89E−5

 R

0.464

0.524

0.464

 Adj. R-square

0.212

0.346

0.272

Avg. motility (µm)

 Mean

96.346 ± 3.49

184.515 ± 7.38***

118.951 ± 4.21*

 Slope

0.778 ± 0.01

1.643 ± 0.01

0.937 ± 0.01

 R

0.998

0.999

0.997

 Adj. R-square

0.997

0.998

0.997

Avg. motility speed (µm/h)

 Mean

96.609 ± 1.46

188.513 ± 3.94***

115.551 ± 2.05

 Slope

0.056 ± 0.02

0.199 ± 0.05

0.033 ± 0.03

 R

0.172

0.227

0.074

 Adj. R-square

0.025

0.047

0.001

  1. Effect of treatment with alizarin (10−5 M) and purpurin (10−5 M)
  2. Data shown in the table represent variables and ± S.D. values calculated for 50 cell/group in 240 consecutive frames
  3. The ‘Mean’ values are expressed as dimensions (μm, μm/h) shown in the sub-headers of the table
  4. The slope values are expressed as dimensionless values to describe the changing rate of Mean values in the 2 h’ time interval of treatments
  5. R and Adj. R-Square represent data of linear regression analysis to describe trends of variables belonging to the parameters calculated
  6. The level of significance is shown as follows: * p < 0.05; ** p < 0.01; *** p < 0.001