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Fig. 2 | Cancer Cell International

Fig. 2

From: Treatment with G-CSF reduces acute myeloid leukemia blast viability in the presence of bone marrow stroma

Fig. 2

G-CSF treatment significantly reduced cell viability of AML blasts in the presence of bone marrow stroma, while CXCR4 expression remained unaffected. Primary patient AML cells were co-cultured with HS-5 human bone marrow stromal cell line and treated with G-CSF at increasing concentrations for 24 h. a Cell viability was measured by live-death discrimination (7-AAD) and volumetric count by flow cytometry. b CXCR4 surface expression was measured by flow cytometry. c HS-5 human bone marrow stromal cells were treated with G-CSF at increasing concentrations for 24 h. Cell viability was measured as for a. *** p < 0.005

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