Fig. 2

G-CSF treatment significantly reduced cell viability of AML blasts in the presence of bone marrow stroma, while CXCR4 expression remained unaffected. Primary patient AML cells were co-cultured with HS-5 human bone marrow stromal cell line and treated with G-CSF at increasing concentrations for 24 h. a Cell viability was measured by live-death discrimination (7-AAD) and volumetric count by flow cytometry. b CXCR4 surface expression was measured by flow cytometry. c HS-5 human bone marrow stromal cells were treated with G-CSF at increasing concentrations for 24 h. Cell viability was measured as for a. *** p < 0.005