Fig. 5
From: Antibiotics inhibit sphere-forming ability in suspension culture
P/S addition does not affect cell cycle but targets ALDH + cells. Cell death (a) and cell cycle (b) were evaluated by flow cytometry analysis on T84 and CPP19 cell lines cultured in suspension condition. Results are the mean ± SEM of triplicate and representative of three separate experiments. c ALDH enzyme activity in T84 and CPP19 cell lines grown in suspension culture was analyzed by flow cytometry. As a negative control, cells were treated with the specific ALDH inhibitor DEAB. The gated cells represent the ALDH positive cells