Fig. 4

Staining MCF-7 cells with trypan blue after treatment with different doses of hypericin for 48 h. a and b MCF-7 cells which had treated with 0.5 (μg/ml) (equal to LD50 for 48 h) became blue after staining (a; ×40 and b; ×100). c MCF-7 cells which had treated with hypericin in higher concentrations than LD50 [25 (μg/ml)] did not become blue (c; ×100)