Fig. 3

2-DG suppresses growth of Kras-driven lung cancer cells with autophagy and apoptosis pathway activation. a–f KP2 and H23 Cell growth were inhibited after treatment of 2-DG. Cell numbers were counted at 48 h after 2.5, 5, 10 and 20 mg/ml 2-DG treatment (a, c) or at 0, 12, 24, 36, 48, 60 and 72 h after 10 mg/ml 2-DG treatment (e, f). Cells treated with 2.5, 5, 10 and 20 mg/ml 2-DG at 48 h were stained with crystal violet in 6-well plate (b, d). g–k Autophagy and apoptosis pathway were activated after 2-DG treatment. g KP2 and H23 cells stably expressing GFP-LC3 were treated with or without 2-DG (10 mg/ml) for 48 h. h, i LC3II and Cleaved caspase3 protein level were detected by immunoblotting in KP2 cells and H23 cells treated with 2.5, 5, 10, 20 mg/ml 2-DG at 48 h. j, k Western blot detected LC3II and Cleaved caspase3 in KP2 and H23 cells treated with 10 mg/ml 2-DG at 0, 12, 24, 36, 48, 60, 72 h