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Table 1 Oxidation of [1-14C]-glucose and [1-14C]-acetate in human tumour cells measured by the release of 14CO2

From: Characterisation of bioenergetic pathways and related regulators by multiple assays in human tumour cells

Cell culture

14CO2 in the cell culture count per 250 s/106 cells

[1-14C]-glucose (A)

[1-14C]-Na-acetate (B)

A/B

HT-1080

11334 ± 2202

377 ± 33

30.1

Fibroblast

3973 ± 684

924 ± 243

4.3

MDA-MB231

3360 ± 584

1344 ± 104

2.5

HepG2

3255 ± 1145

2305 ± 378

1.4

BT747

3081 ± 247

2924 ± 227

1.1

Oscort

5336 ± 171

5580 ± 1240

1.0

ZR-75.1

3450 ± 966

8947 ± 1915

0.4

U937

5948 ± 191

15149 ± 3221

0.4

  1. Tumour cells incubated nutrient-free medium were labelled for 1 h with 0.2 µCi/ml [1-14C]-glucose or [1-14C]-acetate and the released 14CO2 measured. Data from three independent measurements and 3 parallel samples
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Cancer Cell International

ISSN: 1475-2867

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