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Table 2 Concentration of glycolytic and TCA cycle metabolites in tumour cells labelled with [U-13C]-glucose

From: Characterisation of bioenergetic pathways and related regulators by multiple assays in human tumour cells

Metabolites

ZR-75.1

HT-1080

ng/106cells

13C

12C

13C

12C

G6P/F6P

26.3 ± 2.3

10.1 ± 1.3

72.0 ± 7.1

7.4 ± 1.3

Lactate

39.26 ± 1.6

35.9 ± 6.8

61.2 ± 36.0

70.2 ± 12.6

Citrate

14.9 ± 1.7

33.9 ± 4.8

23.2 ± 4.3

33.9 ± 4.5

Succinate

17.1 ± 7.9

11.8 ± 2.3

ND

ND

Malate

28.6 ± 5.9

30.2 ± 1.0

[1-13C]:7.9 ± 2.5

[1-13C]: ND

[2-13C]: 22.2 ± 7.0

[2-13C]: 4.7 ± 0.4

[3-13C]: 23.5 ± 7.2

[3-13C]: 1.9 ± 0.7

[4-13C]: 13.1 ± 2.5

[4-13C]: ND

  1. Tumour cell line: ZR-75.1, HT-1080 Culture medium: D5030
  2. Labelling: [U-13C]-glucose 10 mM for 1 h. Data from three independent measurements and 3 parallel samples