Fig. 7

The detection of autophagy using transmission electron microscope. The A549 cells were treated with 10 µM fisetin (FIS) and/or 0.1 µM paclitaxel (PTX) for 24 h or left untreated (control, CTRL). Arrowheads indicate: (I) empty autophagic-like vacuoles; (II) autophagic-like vacuoles filled with the amorphous materials, the membranous inclusions or the organelles at the various stages of degradation; (III) single-membrane vacuoles (autolysosome-like structures); (IV) the swollen mitochondria with distorted or disorganized cristae; (V) large lysosome-like structure with electron-dense material. Representative micrographs of two independent experiments. Bar 2 µm