Fig. 2From: Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiationChanges in DNA methylation in osteosarcoma cell lines after treatment with MTX. Levels of 5-methylcytosine in Saos-2 (a), OSA-02 (b), OSA-03 (c), OSA-05 (d), OSA-06 (e) and OSA-08 (f) cells as measured using an ELISA assay at day 3 of incubation. The levels of 5-methylcytosine are presented as a percentage change compared to the levels found in untreated control cells. Untreated controls were set as 100 %. The data represent the mean ± SD. Experiments were repeated three times. *P < 0.05, **P < 0.01, indicate significant differences from the respective control groupsBack to article page