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Fig. 2 | Cancer Cell International

Fig. 2

From: Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation

Fig. 2

Changes in DNA methylation in osteosarcoma cell lines after treatment with MTX. Levels of 5-methylcytosine in Saos-2 (a), OSA-02 (b), OSA-03 (c), OSA-05 (d), OSA-06 (e) and OSA-08 (f) cells as measured using an ELISA assay at day 3 of incubation. The levels of 5-methylcytosine are presented as a percentage change compared to the levels found in untreated control cells. Untreated controls were set as 100 %. The data represent the mean ± SD. Experiments were repeated three times. *P < 0.05, **P < 0.01, indicate significant differences from the respective control groups

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