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Table 2 Sequences of the primers used for qPCR

From: Non-DHFR-mediated effects of methotrexate in osteosarcoma cell lines: epigenetic alterations and enhanced cell differentiation

Gene Primer sequence Product length (bp)
RARA F: 5′-CGACCGAAACAAGAAGAAGAAGG-3′
R: 5′-TTCTGAGCTGTTGTTCGTAGTGT-3′
166
RARB F: 5′-TGATGGAGTTGGGTGGACTT-3′
R: 5′-GCTTGGGACGAGTTCCTCAG-3′
288
RXRA F: 5′-CTCAATGGCGTCCTCAAGGT-3′
R: 5′-CACTCCATAGTGCTTGCCTGA-3′
111
RBP1 F: 5′-TGACCGCAAGTGCATGACAA-3′
R: 5′-GACCACACCTTCCACTCTCA-3′
142
CRABP2 F: 5′-TGCTGAGGAAGATTGCTGTG-3′
R: 5′-CCCATTTCACCAGGCTCTTA-3′
183
COLL I F: 5′-CAGACTGGCAACCTCAAGAA-3′
R: 5′-GGAGGTCTTGGTGGTTTTGT-3′
180
ALPL F: 5′-CCACGTCTTCACATTTGGTG-3′
R: 5′-AGACTGCGCCTGGTAGTTGT-3′
196
HSP90AB1 F: 5′-CGCATGAAGGAGACACAGAA-3′
R: 5′-TCCCATCAAATTCCTTGAGC-3′
169