Fig. 3

Suppressing Spry4 expression increases β3-integrin protein level in MDA-MB-231 cells. a Immunoblotting assay shows that S4kd cells had increased β3-integrin levels. b Quantification of β3-integrin protein levels from three independent experiments. c Representative FACS analysis shows that suppressing Spry4 expression increased CD61 positive cells. d Quantification of CD61 positive cell percentages from three independent experiments. e Immunoblotting assay shows that suppressing Spry4 had no effect on pERK and pSrc expression, but increased pAkt expression. Inhibition of MEK/ERK signaling by U0126 or PI3 K/Akt by Ly294002 did not reverse Spry4 knockdown mediated increase of β3-integrin but further increased β3-integrin expression. f Quantification of pERK, pAkt and b3-integrin from three independent immunoblotting assays in E. *p < 0.05; **p < 0.01